Silver Enhancer Kit
SIAL/SE100
Product Type: Chemical
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| application(s) | diagnostic assay manufacturing hematology histology |
| dilution | (for the determine the presence of gold nanorods in various tissues) |
| form | liquid |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| usage | sufficient for ≥100 slides staining and fixing |
| Application: | Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy. |
| Biochem/physiol Actions: | Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required. |
| Features and Benefits: | Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting. |
| Packaging: | The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use. |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H410 |
| Precautionary statements | P273 - P391 - P501 |
| Hazard Codes | N |
| Risk Statements | 51/53 |
| Safety Statements | 61 |
| RIDADR | UN 3082 9 / PGIII |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |
| Components | Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g; Silver enhancer solution A 100 mL; Silver enhancer solution B 100 mL |