Sf-RVN® Insect Cell Line
SIAL/SFRVN
Synonym: Sf9 rhabdovirus-negative (Sf-RVN®) Insect Cell Line
Product Type: Chemical
| description | Sf9 rhabdovirus-negative (Sf-RVN®) Insect Cell Line |
| shipped in | liquid nitrogen |
| solubility | DMSO: 10% (Sf-RVN(R)cells are provided to customers in vials containing 1 mL at 10 x 106 cells/mL. Cells are banked in EX-CELL® CD Insect Cell Medium and 10% DMSO.) |
| storage temp. | −196°C |
| General description: | The Sf-RVN® Insect Cell Line  is a rhabdovirus-negative Sf9 cell line that can be grown in both adherent and suspension culture. Sf-RVN® Insect Cell Line has been adapted for growth in chemically defined medium to be used for the production of recombinant proteins, adeno-associated viruses (AAV) and viral-like particles (VLP). The cell line was tested for adventitious agents and banked under GMP conditions. A technical user guide, with detailed protocols for optimal performances, is provided with the cell line.The EX-CELL® CD Insect Cell Medium , recommended for use, is optimized to get excellent growth and productivity of the Sf-RVN® Insect Cell Line. Combined, these two products form the Sf-RVN® Platform .Cell Line Origin Sf-RVN® is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith and C. Cherry. Sf-RVN® was isolated from Sf9 by GlycoBac, LLC. |
| Legal Information: | EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | EXACT-AIR is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Sf-RVN is a registered trademark of GlycoBac, LLC |
| Other Notes: | Sf-RVN® is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith and C. Cherry. Sf-RVN ® was isolated from Sf9 by GlycoBac, LLC. |
| Physical form: | Sf-RVN® cells are provided to customers in vials containing 1 mL at 10 x 106cells/mL. Cells are banked in EX-CELL® CD Insect Cell Medium and 10% DMSO |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |