ECO TWEEN® 20
SIAL/STS0200 - viscous liquid
Synonym: TWEEN® 20; Polyethylene glycol sorbitan monolaurate; Polyoxyethylenesorbitan monolaurate
CAS Number: 9005-64-5
MDL Number: MFCD00165986
Product Type: Chemical
12 Principles of Green Chemistry: Principle 7—A raw material or feedstock should be renewable rather than depleting whenever technically and economically practicable.
12 Principles of Green Chemistry: Principle 10—Chemical products should be designed so that at the end of their function they break down into innocuous degradation products and do not persist in the environment.
| acid number | ≤2.2 mg KOH/g |
| assay | ≥40.0% (GC) |
| CMC | 0.06 mM (20-25°C) |
| composition | lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids) |
| density | 1.095 g/mL at 25 °C (lit.) |
| description | non-ionic |
| form | viscous liquid |
| greener alternative category | Aligned  |
| greener alternative product characteristics | Use of Renewable Feedstocks Design for Degradation Learn more about the Principles of Green Chemistry . |
| HLB | 16.7 |
| hydroxyl value | 96‑108 mg KOH/g |
| impurities | ≤3.0% water |
| InChI | 1S/C26H50O10/c1-2-3-4-5-6 |
| InChI key | HMFKFHLTUCJZJO-UHFFFAOYSA |
| mol wt | ~1228 |
| Quality Level | 200 |
| refractive index | n |
| transition temp | cloud point 76 °C |
| Application: | Non-ionic detergent |
| Application: | TWEEN 20 has been used as a component of: • washing buffer and blocking buffer in Western blotting • blocking buffer in immunohistochemistry • washing buffer in ELISA (enzyme linked immunosorbent assay) • reaction mixture in PCR (polymerase chain reaction) |
| Application: | TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%. |
| Features and Benefits: | • 100 % Renewable • 100 % Bio-based • Certified to the USDA BioPreferred Program • Lower carbon footprint than petrochemical-based versions |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is a biobased surfactant and is aligned with the 7th principle of Green Chemistry "Use of Renewable Feedstocks" and the 10th principle "Design for Degradation". TWEEN 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Preparation Note: | TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils. |
| Purity | ≥40.0% (GC) |
| Density | 1.095 g/mL at 25 °C (lit.) |
| Refractive Index | n |