Trizma® hydrochloride
SIAL/T15760 - reagent grade, ≥99%, crystalline
Synonym: TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
| assay | ≥99% |
| biological source | synthetic |
| form | crystalline |
| grade | reagent grade |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| pKa (25 °C) | 8.1 |
| Quality Level | 200  |
| SMILES string | Cl.NC(CO)(CO)CO |
| useful pH range | 7.0-9.0 |
| Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. Tris hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure. |
| General description: | Tris hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution. |
| Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% |