Tetramethylrhodamine isothiocyanate Isomer R
SIAL/T3163 - powder
Synonym: 6-TRITC; Tetramethylrhodamine-
CAS Number: 80724-20-5
Empirical Formula (Hill Notation): C25H21N3O3S
Molecular Weight: 443.52
MDL Number: MFCD00063517
Linear Formula: C25H21N3O3S
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3163-10MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3163-5MG
| application(s) | diagnostic assay manufacturing hematology histology |
| composition | Dye content, ≥80% |
| description | labeling efficiency with bovine albumin >= 50% |
| form | powder |
| InChI | 1S/C25H21N3O3S/c1-27(2)16 |
| InChI key | OBYNJKLOYWCXEP-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | CN(C)c1ccc2c(OC3=CC(C=CC |
| solubility | 1 M NH4OH: 10 mg/mL (Faint Red to Very Dark Red to Very Dark Purple solution) |
| storage temp. | 2-8°C |
| Application: | • TRITC is used to label a wide variety of biomolecules, including immunoglobulins, lectins, nucleic acids, polynucleotides, and polysaccharides for affinity, immuno-, and in situ hybridization, fluorescent probes, and flow cytometry. • Lectins are TRITC-Iabeled reagents for the affinity staining of sections and cell monolayers to distinguish the sialomucins of salivary glands. • TRITC-antibodies are used to identify pathogenic amoebae. • TRITC-oligonucleotides are used in the in-situ hybridization staining of soil microorganisms. • TRITC-Iabeled reagents have also been used as fluorescent probes of live cells. • Flow cytometry of TRlTC-Iabeled slime mold cells has been used to study their aggregation behavior. |
| General description: | Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride. |
| Packaging: | 5, 10 mg in glass bottle |
| Hazard statements | H412 |
| Precautionary statements | P273 |
| Risk Statements | 52/53 |
| Safety Statements | 61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |