Tetrazolium Blue Chloride
SIAL/T4375 - used in colorimetric determination of reducing compounds
Synonym: 3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride; 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride); BTC; Blue Tetrazolium chloride; TZ
CAS Number: 1871-22-3
Empirical Formula (Hill Notation): C40H32Cl2N8O2
Molecular Weight: 727.64
EC Number: 217-488-8
MDL Number: MFCD00040933
Linear Formula: C40H32Cl2N8O2
Product Type: Chemical
application(s) | diagnostic assay manufacturing hematology histology |
form | powder |
InChI | 1S/C40H32N8O2.2ClH/c1-49- |
InChI key | MUUHXGOJWVMBDY-UHFFFAOYSA |
mp | 255 °C (dec.) (lit.) |
Quality Level | 200 ![]() |
SMILES string | [Cl-].[Cl-].COc1cc(ccc1-[ |
solubility | methanol: 50 mg/mL, clear |
storage temp. | room temp |
Application: | Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test. |
Packaging: | 1, 5, 10, 25 g in glass bottle |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H350 |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
Hazard Codes | T |
Risk Statements | 45 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
mp | 255 °C (dec.) (lit.) |
Storage Temp. | room temp |
UNSPSC | 12171500 |