Trichloroacetic acid
SIAL/T6399 - ACS reagent, ≥99.0%
Synonym: TCA
CAS Number: 76-03-9
Empirical Formula (Hill Notation): C2HCl3O2
Molecular Weight: 163.39
EC Number: 200-927-2
MDL Number: MFCD00004177
Linear Formula: Cl3CCOOH
Product Type: Chemical
| anion traces | chloride (Cl-): ≤0.002% |
| nitrate (NO3-): ≤0.002% | |
| phosphate (PO43-): ≤5 ppm | |
| sulfate (SO42-): ≤0.02% | |
| assay | ≥99.0% |
| bp | 196 °C (lit.) |
| cation traces | Fe: ≤0.001% |
| heavy metals (as Pb): ≤0.002% | |
| density | 1.62 g/mL at 25 °C (lit.) |
| form | crystalline |
| functional group | carboxylic acid |
| chloro | |
| grade | ACS reagent |
| ign. residue | ≤0.03% |
| impurities | Substances darkened by sulfuric acid, passes test |
| ≤0.01% insoluble matter | |
| InChI | 1S/C2HCl3O2/c3-2(4,5)1(6) |
| InChI key | YNJBWRMUSHSURL-UHFFFAOYSA |
| mp | 54-58 °C (lit.) |
| pH | (1 at 81.7 g/l at 25 °C) |
| Quality Level | 200 ![]() |
| refractive index | n |
| SMILES string | OC(=O)C(Cl)(Cl)Cl |
| solubility | H2O: 1 g/10 mL |
| storage temp. | 2-8°C |
| vapor density | <1 (vs air) |
| vapor pressure | 1 mmHg ( 51 °C) |
| Application: | • Trichloroacetic acid has been used for the precipitation of sLRP (soluble circulating low density lipoprotein receptor-related protein-1) isolated from plasma. • It has been used for the extraction of ascorbic acid from plant samples. It has also been used for the preparation of ascorbate standards for ascorbate assay. • It has been used for the precipitation of IMAC (immobilized metal ion affinity chromatography)-isolated phosphoproteins. |
| Application: | Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy. |
| Packaging: | 1 kg in glass bottle |
| Packaging: | 5, 100, 4×100, 250, 500 g in glass bottle |
| Symbol | ![]() ![]() GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H314 - H335 - H410 |
| Precautionary statements | P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | C,N |
| Risk Statements | 35-50/53 |
| Safety Statements | 26-36/37/39-45-60-61 |
| RIDADR | UN 1839 8 / PGII |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% |
| bp | 196 °C (lit.) |
| mp | 54-58 °C (lit.) |
| Density | 1.62 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352100 |




