Tryptic Soy Broth
SIAL/T8907 - Microbiologically tested., NutriSelect® Basic
Synonym: Soybean Casein digest Broth, Casein Soya Broth, CASO Broth, TSB; CASO Broth; Casein Soya Broth; Soybean Casein digest Broth; TSB; Tryptone Soya Broth
MDL Number: MFCD00132536
Product Type: Chemical
Microbial culture media preparation
| application(s) | environmental food and beverages microbiology pathogen testing pharmaceutical |
| form | powder |
| manufacturer/tradename | NutriSelect® Basic |
| packaging | poly bottle of 1 kg |
| poly bottle of 500 g | |
| quality | Microbiologically tested. |
| Quality Level | 200  |
| sterility | non-sterile |
| suitability | bacteria |
| Application: | Tryptic Soy Broth is a widely used medium for culturing aerobic gram-positive, gram-negative bacteria, fungus, routine blood cultures, and microbial biofilms. It is also been used as a growth medium to culture Staphylococcus aureus strains and as a growth medium for E.coli in the bacterial-killing assay (BKA) with bird plasma samples. |
| Components: | Casein (pancreatic digest) 17 g/L Soya peptone (papaic digest) 3 g/L Sodium chloride 5 g/L Dipotassium phosphate 2.5 g/L Dextrose 2.5 g/L |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Tryptic Soy Broth is a general-purpose liquid medium used in qualitative procedures for the sterility testing, enrichment, and cultivation of fastidious microorganisms. The formulation contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids and long-chained peptides. Sodium chloride maintains the osmotic equilibrium and dextrose serves as a source of carbohydrate and energy. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 500 g in poly bottle |
| Preparation Note: | Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171605 |