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Triton X-100

SIAL/T9284 - BioXtra

Synonym: t-Octylphenoxypolyethoxyethanol; Polyethylene glycol tert-octylphenyl ether

CAS Number: 9036-19-5
MDL Number: MFCD00128254
Linear Formula: t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-T9284-100ML 100 mL
$91.10
1/EA
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45-T9284-500ML 500 mL
$130.00
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45-T9284-1L 1 L
$190.00
1/EA
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45-T9284-10L 10 L
$1230.00
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aggregation number 100-155
anion traces chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  Na: ≤0.1%
  NH4+: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
CMC 0.2-0.9 mM (20-25°C)
description non-ionic
form liquid
HLB 13.5
ign. residue ≤0.4%
impurities ≤0.05% Phosphorus (P)
InChI 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key HNLXNOZHXNSSPN-UHFFFAOYSA-N
mol wt average mol wt 625
  micellar avg mol wt 80,000
product line BioXtra
Quality Level 200 
SMILES string CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
solubility water: soluble
technique(s) permeability assay: suitable
transition temp cloud point 65 °C
  pour point ~7 °C
Application: Triton X-100 has been used:
• Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
• As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
• In the blocking solution during immunocytochemistry procedure
• During deglycosylation procedure
• For membrane solubilization
• For cell permeabilization
• In decellularization of porcine heart valve tissue
Biochem/physiol Actions: Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
General description: Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Legal Information: Span is a registered trademark of Croda International PLC
Legal Information: Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Legal Information: TWEEN is a registered trademark of Croda International PLC
Packaging: 1 L in poly bottle
Packaging: 100, 500 mL in poly bottle
UNSPSC 12161900

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