Triton™ X-100
SIAL/T9284 - BioXtra
Synonym: t-
CAS Number: 9036-19-5
Empirical Formula (Hill Notation): (C2H4O)nC14H22O
MDL Number: MFCD00128254
Linear Formula: t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Product Type: Chemical
| aggregation number | 100-155 |
| anion traces | chloride (Cl-): ≤0.05% |
| sulfate (SO42-): ≤0.05% | |
| biological source | synthetic |
| cation traces | Al: ≤0.0005% |
| Ca: ≤0.0005% | |
| Cu: ≤0.0005% | |
| Fe: ≤0.0005% | |
| K: ≤0.005% | |
| Mg: ≤0.0005% | |
| Na: ≤0.1% | |
| NH4+: ≤0.05% | |
| Pb: ≤0.001% | |
| Zn: ≤0.0005% | |
| CMC | 0.2-0.9 mM (20-25°C) |
| density | 1.065 g/cm3 at 20 °C (68 °F) |
| description | non-ionic |
| form | liquid |
| HLB | 13.5 |
| ign. residue | ≤0.4% |
| impurities | ≤0.05% Phosphorus (P) |
| InChI | 1S/C28H50O8/c1-27(2,3)24- |
| InChI key | HNLXNOZHXNSSPN-UHFFFAOYSA |
| mol wt | average mol wt 625 |
| micellar avg mol wt 80,000 | |
| product line | BioXtra |
| Quality Level | 200 ![]() |
| SMILES string | CC(C)(C)CC(C)(C)c1ccc(OCC |
| solubility | water: soluble |
| technique(s) | permeability assay: suitable |
| transition temp | cloud point 65 °C |
| pour point ~7 °C |
| Application: | Triton™ X-100 has been used: • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling • In the blocking solution during immunocytochemistry procedure • During deglycosylation procedure • For membrane solubilization • For cell permeabilization • In decellularization of porcine heart valve tissue |
| Biochem/physiol Actions: | Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. |
| Features and Benefits: | • BioXtra Grade product for your Cell Biology and Biochemical research • Non-ionic nondenaturing detergent • Superior wetting agent and emulsifier • Highly versatile surfactant |
| General description: | Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection. |
| Legal Information: | Span is a registered trademark of Croda International PLC |
| Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research ![]() |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 1 L in poly bottle |
| Packaging: | 100, 500 mL in poly bottle |
| Symbol | ![]() ![]() GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H410 |
| Precautionary statements | P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn,N |
| Risk Statements | 22-36-51/53 |
| Safety Statements | 26-39-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 483.8 °F |
| Flash Point(C) | 251 °C |
| Density | 1.065 g/cm3 at 20 °C (68 °F) |
| UNSPSC | 12161900 |




