VirusExpress® 293T Lentiviral Production Cells
SIAL/VP001
Synonym: HEK293T cells for lentivirus production
Product Type: Chemical
| Quality Level | 600  |
| shipped in | liquid nitrogen |
| storage temp. | −196°C |
| General description: | VirusExpress 293T Lentiviral Production Cells are a clonal, suspension-adapted HEK293T derivative selected for fast growth, high transfection efficiency, and high-titer lentivirus production. VirusExpress 293T Lentiviral Production Cells grow to high cell densities, exhibit minimal cell clumping, and are amenable to PEI-based transfection. A medium exchange step is not needed prior to transfection with a PEI transfection reagent, and the cells can be transfected in larger volumes, enabling scale-up of lentivirus production. The cells are intended to be cultured in, and can be directly thawed into EX-CELL® CD HEK293 Viral Vector Medium. VirusExpress 293T Lentiviral Production Cells are derived from the HEK293T cell line (ATCC CRL-3216) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham FL et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. HEK293T cells were created by stable transfection of the HEK293 cell line with a temperature-sensitive mutant of SV40 large T antigen. Due to the expression of the large T antigen, transfected plasmids that carry the SV40 origin of replication can replicate in 293T cells and will transiently maintain a high copy number. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs. |
| Legal Information: | A commercial license must be taken prior to use of the cells, or any products or materials derived from the cells, in a manufacturing process. Please contact your local sales representative for more details. |
| Legal Information: | EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | The cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL. The cells were banked using under cGMP conditions in chemically defined medium supplement with 6 mM glutamine and 8% DMSO. |
| Packaging: | This product is shipped in a liquid nitrogen dry vapor shipper. The liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |