VirusExpress® 293 AAV Production Cells
SIAL/VP002
Synonym: HEK293 cells for AAV production
Product Type: Chemical
| Quality Level | 600  |
| shipped in | liquid nitrogen |
| storage temp. | −196°C |
| General description: | VirusExpress® 293 AAV Production Cells are a clonal, suspension-adapted HEK293 derivative selected for fast growth, high transfection efficiency, and high-titer AAV production. VirusExpress 293 AAV Production Cells grow to high cell densities and are amenable to chemical-based transfection reagents commonly used in manufacturing (i.e., PEI, lipid-nanoparticle). A medium exchange step is not needed prior to transfection, and the cells can be transfected in larger volumes, enabling scale-up of AAV production. The cells are supplied in a chemically defined media formulation and can be thawed directly into EX-CELL® CD HEK293 Viral Vector Medium. VirusExpress 293 AAV Production Cells are derived from the HEK293 cell line (ATCC CRL-1573) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham F.L. et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs. |
| Legal Information: | A commercial license must be taken prior to use of the cells, or any products or materials derived from the cells, in a manufacturing process. Please contact your local sales representative for more details. |
| Legal Information: | EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | The cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL. The cells were banked under cGMP conditions in chemically defined medium and 8% DMSO. |
| Packaging: | This product is shipped in a liquid nitrogen dry vapor shipper. The liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. |
| Storage Temp. | −196°C |