Wright stain
SIAL/W0625 - suitable for blood stain
Synonym: Eosin Methylene blue according to Wright
CAS Number: 68988-92-1
MDL Number: MFCD00082143
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: W0625-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: W0625-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: W0625-25G
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≤4% (HPLC) |
| color | dark green |
| concentration | ≤4% (HPLC) |
| form | powder |
| InChI | 1S/C20H8Br4O5.C16H18N3S/c |
| InChI key | AXIKDPDWFVPGOD-UHFFFAOYSA |
| Quality Level | 200  |
| solubility | methanol: 10 mg/10 mL |
| storage temp. | room temp |
| suitability | suitable for blood stain |
| Application: | Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.• It is also used for G-banding and helps in the analysis of poorly spread metaphases. • It has been used to study the cytogenetics of human hematopoietic cells. • It has also been employed in the karyotyping of human embryonic stem cells. • It is also used in the staining of chromosomes. • It is used for the exudate analysis of white blood cell concentration. |
| Application: | Reported to be superior to May-Grunwald Stain in blood staining. |
| Features and Benefits: | The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses. |
| General description: | Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes. |
| Packaging: | 10, 25, 100 g in glass bottle |
| Preparation Note: | Prepared using polychromed (oxidized) methylene blue and eosin Y. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 - H319 |
| Precautionary statements | P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 22-41 |
| Safety Statements | 26-39 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≤4% (HPLC) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |