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Sheepshead minnow (Cyprinodon variegatus), 48-hr Acute, nonrenewal, 25°C, SSW

SIAL/WET046 - Proficiency Testing Material

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-PEWET046-1EA 1 ea
$148.00
1/EA
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application(s) environmental
concentration in water
format matrix material
packaging ampule of 14 × 2 mL
Quality Level 100 
Application: Sheepshead minnow (Cyprinodon variegatus), 48-hr Acute, nonrenewal, 25°C, SSW proficiency testing material is designed for use in chronic Whole Effluent Toxicity (WET) proficiency testing.

Have any questions? Explore our FAQs .
For more information on this product, please contact our PT Service Team .
Features and Benefits: We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory.

Our proficiency testing (PT) program is a benchmark to:

• provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process
• provide a comparison of performance with that of other laboratories, nationally and internationally
• test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems
• provide an independent evaluation of laboratory performance
• demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality

General description: This Whole Effluent Toxicity (WET) proficiency testing sample is produced in accordance with ISO/IEC 17043:2010. This sample is manufactured according to TNI requirements. However, please note, this sample may include non-TNI analytes as well.

In the acute WET test, Sheepshead minnow is employed as a test organism and is exposed to various effluent concentrations. The toxicity of wastewater effluents is estimated for a specific time period. This method is used to test the effect of effluents in wastewaters, which have a negative impact on the aquatic ecosystems.

Supelco® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs. Our PT program is designed to meet the quality assurance requirements in compliance with Discharge Monitoring Report - Quality Assurance Program (DMR-QA), thereby ensuring the analytical competence of your laboratory.

Analyte/s: Values of analytes vary lot to lot.

All information regarding the use of Sheepshead minnow (Cyprinodon variegatus), 48-hr Acute, nonrenewal, 25°C, SSW proficiency testing standard can be found in the reporting packet which will be available to download at PT Portal .
Legal Information: Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Are you new to Supelco® Proficiency Testing? Be sure to register your account details  in the PT Portal before placing your order.
This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at ptservice@milliporesigma.com.

When placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page.
Click here  to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.

Proficiency testing samples are presented as single blind. No values for the samples are released until the testing period concludes.

Whole Effluent Toxicity (WET) tests are designed to predict the impact and toxicity of effluents discharges.

Proficiency Testing Schedule 2023

DMR-QA (US customers only)
DMR-QA 43 2023 Dates TBD

Quick-Turn Study (on demand proficiency test that is offereddepending on lot availability)
Preparation Note: Description

•  The effluent is provided as a concentrate for dilution in synthetic seawater.
•  This concentrate is an EPA-specified toxicant in reagent-grade DI water.
•  Enough material has been provided to run the method at least twice.
•  Do not deviate from the supplied instructions or EPA Method 2004.0.
•  Contact ptservice@milliporesigma.com before running the test if you see the need to deviate from the test conditions.
•  Deviations may affect your final evaluation.

Preparation Instructions

•  To prepare 1 L of effluent, add 900 mL of synthetic seawater to a volumetric flask.
•  Preparation instructions for synthetic seawater can be found in the EPA document EPA-821-R-02-012, page 31.
•  Transfer the entire contents of one container to the flask.
•  Rinse the container with synthetic seawater and transfer to the volumetric flask. Bring flask to 1 L volume with synthetic seawater.
•  This represents 1 L of effluent water for the test.

Required Test Condition

•  Test Type: Static non-renewal
•  Test duration: 48 Hours
•  Temperature: 25 ºC ±1 ºC. The test must not deviate by more than 3 ºC during the test.
•  Renewal of Test solutions: non-renewal
•  Dilution water: Synthetic seawater

Test Conditions

•  Light quality: Ambient laboratory illumination (recommended)
•  Light intensity: 10-20 μE/m2/s (50-100 ft-c) (ambient laboratory levels) (recommended)
•  Photoperiod: 16 h light, 8 h darkness (recommended)
•  Test chamber size: 250 mL (recommended minimum)
•  Test solution volume: 200 mL (recommended minimum)
•  Renewal of test solutions: After 48 h (required minimum)
•  Age of test organisms: 1-14 days; less than or equal to 24-h range in age (required)
•  No. of organisms per test chamber: 10 for effluent and receiving water tests (required minimum)
•  No. of replicate chambers per concentration: 2 for effluent tests (required minimum) 4 for receiving water tests (required minimum)
•  No. of organisms per concentration: 20 for effluent tests (required minimum) 40 for receiving water tests (required minimum)
•  Feeding regime: Artemia nauplii are made available while holding prior to the test
•  Test chamber cleaning: Cleaning not required
•  Test solution aeration: None, unless DO concentration falls below 4.0 mg/L; rate should not exceed 100 bubbles/min (recommended)
•  Test concentrations: Effluents: 5 and a control (required minimum)
•  Endpoint: Effluents: Mortality (required)
•  Test acceptability criterion: 90% or greater survival in controls (required).
Storage and Stability: • Please refer to sample label for expiration date, specific storage temperature, and specific storage conditions.
• If storage conditions are not provided on the label, it is intended for room temperature storage.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
UNSPSC 41116107

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