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Triton X-100

SIAL/X100 - laboratory grade

Synonym: t-Octylphenoxypolyethoxyethanol; Polyethylene glycol tert-octylphenyl ether

CAS Number: 9036-19-5
MDL Number: MFCD00128254
Linear Formula: t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-X100-5ML 5 mL
$17.90
1/EA
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45-X100-100ML 100 mL
$54.40
1/EA
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45-X100-500ML 500 mL
$68.90
1/EA
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45-X100-6X500ML 500 mL
$285.00
6/EA
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45-X100-1L 1 L
$107.61
1/EA
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45-X100-1GA 1 gal
$264.00
1/EA
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45-X100-5GA 5 gal
$810.00
1/EA
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aggregation number 100-155
CMC 0.2-0.9 mM (20-25°C)
description non-ionic
form liquid
grade laboratory grade
HLB 13.5
InChI 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key HNLXNOZHXNSSPN-UHFFFAOYSA-N
mol wt average mol wt 625
  micellar avg mol wt 80,000
Quality Level 200 
SMILES string CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
solubility water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
transition temp cloud point 65 °C
  pour point ~7 °C
Application: Triton-X has been used:
• In the permeabilization of cells for immunofluorescence staining
• As a component of lysis buffer in western blot analysis
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope
Biochem/physiol Actions: Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
General description: Triton X-100 has hemolytic property and is used for DNA extraction.
General description: Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Legal Information: Tri-Chek is a trademark of Micro Essential Laboratory, Inc.
Legal Information: Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Packaging: 1 gal in poly bottle
Packaging: 1 L in poly bottle
Packaging: 100, 500 mL in poly bottle
Packaging: 5 gal in poly drum
Packaging: 5 mL in glass bottle
UNSPSC 12161900

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