Chloroform
SIGALD/650498 - HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Synonym: Methylidyne trichloride; Trichloromethane
CAS Number: 67-66-3
Empirical Formula (Hill Notation): CHCl3
Molecular Weight: 119.38
EC Number: 200-663-8
MDL Number: MFCD00000826
Linear Formula: CHCl3
Product Type: Chemical
λ | H2O reference |
application(s) | food and beverages |
assay | ≥99.9% |
bp | 60.5-61.5 °C (lit.) |
contains | amylenes as stabilizer |
density | 1.48 g/mL at 25 °C |
1.492 g/mL at 25 °C (lit.) | |
evapn. residue | ≤0.0001% |
form | liquid |
grade | for residue analysis |
HPLC Plus | |
halogenated residue | <10 ng/L (as heptachlor epoxide) |
impurities | ≤0.02% water |
≤0.5 ppb Fluorescence (quinine) at 365 nm | |
InChI | 1S/CHCl3/c2-1(3)4/h1H |
InChI key | HEDRZPFGACZZDS-UHFFFAOYSA |
mp | −63 °C (lit.) |
Quality Level | 100 ![]() |
refractive index | n |
SMILES string | ClC(Cl)Cl |
technique(s) | gas chromatography (GC): suitable |
HPLC: suitable | |
UV absorption | λ: 245 nm Amax: 1.00 |
λ: 250 nm Amax: 0.30 | |
λ: 275-400 nm Amax: 0.005 | |
vapor density | 4.1 (vs air) |
vapor pressure | 160 mmHg ( 20 °C) |
Application: | Chloroform may be used in the following: • Extraction of active constituents of anti-inflammatory drugs for their analysis by attenuated total reflectance-Fourier transform infrared spectroscopic (ATR-FTIR) methods • Estimation of ascorbic acid, free phenolic acids, and flavonoids in extracts of chloroform, methanol, water and, acetic acid obtained from two edible leaf samples using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector (DAD) • Preparation of cannabis product extracts for the analysis of delta-9-tetrahydrocannabi • Investigation of different solvents to measure their recovery ratios for the extraction of acrylamide and 5-hydroxymethylfurfural (HMF) from seafood samples to develop an HPLC-MS/MS-based method for their quantification • Extraction of pesticides by direct-immersion single drop micro-extraction (SDME) method from mango samples for their subsequent analysis by GC-MS • Sample preparation in the profiling of lipids from complex biological samples using off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry |
Application: | Chloroform was used in the making of silanized coverslips for DNA combing. It may be used as: • Extractant in solvent extraction process. • A component of the binary mobile phases for liquid chromatography–atmospheri • Solvent for recrystallization. |
Application: | Suitable for HPLC, spectrophotometry, environmental testing |
General description: | Chloroform is an organic chlorinated hydrocarbon (CHC) commonly used in industries as a solvent. |
Packaging: | 1, 6×1, 4, 4×4 L in glass bottle |
Recommended products: | Discover LiChropur reagents ideal for HPLC ![]() ![]() |
Symbol | ![]() ![]() |
Signal word | Danger |
Hazard statements | H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 |
Precautionary statements | P202 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313 |
Hazard Codes | Xn |
Risk Statements | 63-20-22-36/38-40-48/20/22-67 |
Safety Statements | 26-36/37 |
RIDADR | UN 1888 6.1 / PGIII |
WGK Germany | WGK 3 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Purity | ≥99.9% |
bp | 60.5-61.5 °C (lit.) |
mp | −63 °C (lit.) |
Density | 1.48 g/mL at 25 °C; 1.492 g/mL at 25 °C (lit.) |
Refractive Index | n |
UNSPSC | 12190000 |