MDCK-II Cell Line canine
SIGMA/00062107
Synonym: MDCK Type II Cells; MDCK-2 Cells; MDCKII-WT Cells
Product Type: Chemical
| biological source | canine kidney |
| description | Canine Cocker Spaniel Kidney |
| form | liquid |
| growth mode | Adherent |
| karyotype | Dog |
| morphology | Polarised epithelial |
| products | Not specified |
| Quality Level | 100  |
| receptors | Not specified |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Cell Line Description: | MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (Sigma catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC. |
| Cell Line Origin: | Canine Cocker Spaniel Kidney |
| Culture Medium: | EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 5% FBS (F2442) |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:5 twice a week i.e. seeding at 5 x 10,000 cells/cm2 using 0.25% tryspin or trypsin/EDTA; 5% CO2 37°C, |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |