MDCK-II Cell Line canine

SIGMA/00062107

Synonym: MDCK Type II Cells; MDCK-2 Cells; MDCKII-WT Cells

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-00062107-1VL	1 vial	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

biological source	canine kidney
description	Canine Cocker Spaniel Kidney
form	liquid
growth mode	Adherent
karyotype	Dog
morphology	Polarised epithelial
products	Not specified
Quality Level	100
receptors	Not specified
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Cell Line Description:	MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (Sigma catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm². This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC.
Cell Line Origin:	Canine Cocker Spaniel Kidney
Culture Medium:	EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 5% FBS (F2442)
Other Notes:	Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Subculture Routine:	Split sub-confluent cultures (70-80%) 1:5 twice a week i.e. seeding at 5 x 10,000 cells/cm² using 0.25% tryspin or trypsin/EDTA; 5% CO₂ 37°C,