Nancy-520
SIGMA/01494 - suitable for RT-PCR, ≥95.0% (HPCE)
EC Number: 200-664-3
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| assay | ≥95.0% (HPCE) |
| fluorescence | λex 520 nm; λem 554 nm in TE buffer; DNA |
| form | liquid |
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 100 |
| solubility | DMSO: soluble |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| Application: | Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA. In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520 Nancy-520 has been used successfully in PCR (Real time-PCR) applications. Nancy 520 Greener alternative to Ethidium bromide. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| WGK Germany | WGK 2 |
| Flash Point(F) | 188.6 °F - closed cup - Sol |
| Flash Point(C) | 87 °C - closed cup - Solven |
| Purity | ≥95.0% (HPCE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |