Nancy-520

SIGMA/01494 - suitable for RT-PCR, ≥95.0% (HPCE)

EC Number: 200-664-3
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-01494-500UL	500 µL	$184.00 1/EAAdd To Favorites		Add To Favorites
45-01494-5ML	5 mL	$2060.00 1/EAAdd To Favorites		Add To Favorites
45-01494-10ML	10 mL	$3060.00 1/EAAdd To Favorites		Add To Favorites

12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.

assay	≥95.0% (HPCE)
fluorescence	λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA
form	liquid
greener alternative category	Aligned ,
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry .
Quality Level	100
solubility	DMSO: soluble
storage temp.	2-8°C
sustainability	Greener Alternative Product

Application:	Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA. In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520 Nancy-520 has been used successfully in PCR (Real time-PCR) applications. Nancy 520 Greener alternative to Ethidium bromide.
General description:	We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.