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Atto 520-Biotin

SIGMA/01632 - BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym: Biotin-Atto 520

Empirical Formula (Hill Notation): C37H53ClN6O8S
Molecular Weight: 777.37
MDL Number: MFCD04040671
Linear Formula: C37H53ClN6O8S
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-01632-1MG-F 1 mg
 $115.00
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λ in ethanol (with 0.1% trifluoroacetic acid)
assay ≥90% (HPLC)
form powder
InChI 1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;
InChI key OCYRRKMPTKPPHX-QITHAKLPSA-N
manufacturer/tradename ATTO-TEC GmbH
product line BioReagent
Quality Level 100 
SMILES string [O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]CC)C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C
solubility DMF: soluble
  ethanol: soluble
  methanol: soluble
storage temp. 2-8°C
suitability suitable for fluorescence
UV absorption λ: 522-528 nm Amax
Application: Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke’s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.
Legal Information: This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥90% (HPLC)
Storage Temp. 2-8°C
UNSPSC 12352200

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