Atto 520-Biotin

SIGMA/01632 - BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym: Biotin-Atto 520

Empirical Formula (Hill Notation): C₃₇H₅₃ClN₆O₈S
Molecular Weight: 777.37
MDL Number: MFCD04040671
Linear Formula: C₃₇H₅₃ClN₆O₈S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-01632-1MG-F	1 mg	$119.00 1/EAAdd To Favorites	Add To Favorites

λ	in ethanol (with 0.1% trifluoroacetic acid)
assay	≥90% (HPLC)
form	powder
InChI	1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;
InChI key	OCYRRKMPTKPPHX-QITHAKLPSA-N
manufacturer/tradename	ATTO-TEC GmbH
product line	BioReagent
Quality Level	100
SMILES string	[O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]CC)C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C
solubility	DMF: soluble
	ethanol: soluble
	methanol: soluble
storage temp.	2-8°C
suitability	suitable for fluorescence
UV absorption	λ: 522-528 nm A_max

Application:

Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke’s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.

Legal Information:

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90% (HPLC)
Storage Temp.	2-8°C
UNSPSC	12352108