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Acriflavine

SIGMA/01673 - BioReagent, suitable for fluorescence, ≥90% (AT)

Synonym: 3,6-Diamino-10-methylacridinium chloride mixt. with 3,6-diaminoacridine (proflavine); Euflavine; Trypaflavine Neutral

CAS Number: 8048-52-0
Empirical Formula (Hill Notation): C14H14ClN3
Molecular Weight: 259.73
MDL Number: MFCD00064307
Linear Formula: C14H14ClN3
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-01673-10G 10 g
 $73.90
1/EA		 Add To Favorites
45-01673-50G 50 g
 $220.00
1/EA		 Add To Favorites

 

assay ≥90% (AT)
composition Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC
fluorescence λex 463 nm; λem 490 nm in ethanol
form powder
InChI 1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H
InChI key PEJLNXHANOHNSU-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string [Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46
solubility H2O: 0.33 g/mL (lit.)(lit.)
suitability suitable for fluorescence
Application: Acriflavine is used to fluorescently label high molecular weight RNA molecules .
Excitation and emission wavelengths in various solvents :
• Methanol: λex = 424 nm; λem = 518 nm
• Ethanol: λex = 426 nm; λem = 524 nm
• Propanol: λex = 430 nm; λem = 512 nm
• Butanol: λex = 430 nm; λem = 526 nm
• Formamide: λex = 434 nm; λem = 524 nm
• Glycerol: λex = 432 nm; λem = 540 nm
• Water: λex = 416 nm; λem = 514 nm

Insoluble in ether, chloroform, and fixed oils. Utilized in fluorescence steady state measurements as a donor molecule (when paired with rhodamine 6G as the acceptor) to function as a pH sensor .
Other Notes: DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously
Purity ≥90% (AT)
UNSPSC 12171500

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