Acriflavine
SIGMA/01673 - suitable for fluorescence, BioReagent, ≥90% (AT)
Synonym: 3,6-
CAS Number: 8048-52-0
Empirical Formula (Hill Notation): C14H14ClN3
Molecular Weight: 259.73
MDL Number: MFCD00064307
Linear Formula: C14H14ClN3
Product Type: Chemical
| assay | ≥90% (AT) |
| composition | Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacri |
| fluorescence | λex 463 nm; λem 490 nm in ethanol |
| form | powder |
| InChI | 1S/C14H13N3.C13H11N3.ClH/ |
| InChI key | PEJLNXHANOHNSU-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | [Cl-].Nc1ccc2cc3ccc(N)cc3 |
| solubility | H2O: 0.33 g/mL (lit.)(lit.) |
| suitability | suitable for fluorescence |
| Application: | Acriflavine is used to fluorescently label high molecular weight RNA molecules . Excitation and emission wavelengths in various solvents : • Methanol: λex = 424 nm; λem = 518 nm • Ethanol: λex = 426 nm; λem = 524 nm • Propanol: λex = 430 nm; λem = 512 nm • Butanol: λex = 430 nm; λem = 526 nm • Formamide: λex = 434 nm; λem = 524 nm • Glycerol: λex = 432 nm; λem = 540 nm • Water: λex = 416 nm; λem = 514 nm Insoluble in ether, chloroform, and fixed oils. Utilized in fluorescence steady state measurements as a donor molecule (when paired with rhodamine 6G as the acceptor) to function as a pH sensor . |
| Other Notes: | DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H302 - H315 - H319 - H335 - H400 |
| Precautionary statements | P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn,N |
| Risk Statements | 22-36/37/38-50 |
| Safety Statements | 26-61 |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (AT) |
| UNSPSC | 12171500 |