N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
SIGMA/03449 - ≥99.0% (AT)
Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride; EDAC; EDC; EDC hydrochloride; WSC hydrochloride
CAS Number: 25952-53-8
Empirical Formula (Hill Notation): C8H17N3 · HCl
Molecular Weight: 191.70
EC Number: 247-361-2
MDL Number: MFCD00012503
Linear Formula: C8H17N3 · HCl
Product Type: Chemical
| assay | ≥99.0% (AT) |
| form | powder |
| InChI | 1S/C8H17N3.ClH/c1-4-9-8-1 |
| InChI key | FPQQSJJWHUJYPU-UHFFFAOYSA |
| mp | 110-115 °C (lit.) |
| 112-116 °C | |
| Quality Level | 200  |
| SMILES string | Cl.CCN=C=NCCCN(C)C |
| solubility | H2O: soluble 0.2 g/L |
| storage temp. | −20°C |
| Application: | N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used for amine coupling reaction during self-assembly of the tethered bilayer lipid membrane. It has also been used for derivatizing antibody against COX5b to carboxylated polystyrene microspheres. |
| Application: | N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride ≥99.0% (AT) has been used as an activator to covalently immobilize tyrosinase to prepare enzyme electrodes. It has also been used as a prefixative for histamine immunolabeling. |
| Biochem/physiol Actions: | N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it will react with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates. |
| Biochem/physiol Actions: | Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided. |
| Other Notes: | Water soluble peptide coupling reagent; Modification of carboxyl groups in proteins, review; Synthesis of esters. |
| Symbol |    GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H311 - H315 - H317 - H373 - H410 |
| Precautionary statements | P260 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P314 |
| Hazard Codes | Xi |
| Risk Statements | 37/38-41 |
| Safety Statements | 26-36/37/39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (AT) |
| mp | 110-115 °C (lit.); 112-116 °C |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |