Ethylenediaminetetraacetic acid tetrasodium salt hydrate

SIGMA/03699 - BioUltra, ≥99.0% (KT)

Synonym: EDTA tetrasodium salt; Edathamil; Tetrasodium ethylenediaminetetraacetate hydrate

CAS Number: 194491-31-1
Empirical Formula (Hill Notation): C₁₀H₁₂N₂Na₄O₈ · xH₂O
Molecular Weight: 380.17 (anhydrous basis)
EC Number: 200-573-9
MDL Number: MFCD00150025
Linear Formula: (NaOOCCH₂)₂NCH₂CH₂N(CH₂COONa)₂ · xH₂O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-03699-250G	250 g	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-03699-1KG	1 kg	$364.00 1/EAAdd To Favorites	Add To Favorites

λ	0.1 M in H₂O
anion traces	chloride (Cl^-): ≤100 mg/kg
	sulfate (SO₄^2-): ≤100 mg/kg
assay	≥99.0% (KT)
cation traces	Al: ≤5 mg/kg
	As: ≤0.1 mg/kg
	Ba: ≤5 mg/kg
	Bi: ≤5 mg/kg
	Ca: ≤10 mg/kg
	Cd: ≤5 mg/kg
	Co: ≤5 mg/kg
	Cr: ≤5 mg/kg
	Cu: ≤5 mg/kg
	Fe: ≤5 mg/kg
	K: ≤50 mg/kg
	Li: ≤5 mg/kg
	Mg: ≤5 mg/kg
	Mn: ≤5 mg/kg
	Mo: ≤5 mg/kg
	Ni: ≤5 mg/kg
	Pb: ≤5 mg/kg
	Sr: ≤5 mg/kg
	Zn: ≤5 mg/kg
form	powder
impurities	insoluble matter, passes filter test
InChI	1S/C10H16N2O8.4Na.H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;1H2/q;4*+1;/p-4
InChI key	GXFFSKMBMYHTAE-UHFFFAOYSA-J
pH	11.0-12.0 (25 °C, 0.1 M in H₂O)
product line	BioUltra
Quality Level	200
reaction suitability	reagent type: chelator
SMILES string	O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O
solubility	H₂O: 0.1 M at 20 °C, clear, colorless
UV absorption	λ: 260 nm A_max: ≤1.5
	λ: 280 nm A_max: ≤0.06

Application:

EDTA is an inhibitor of metalloproteases, at effective concentrations of 1-10 mM. EDTA acts as a chelator of the zinc ion in the active site of metalloproteases, and can also inhibit other metal ion-dependent proteases such as calcium-dependent cysteine proteases. For use as an anticoagulant, disodium or tripotassium salts of EDTA are most commonly used. The optimal concentration is 1.5 mg per ml of blood.

Preparation Note:

Soluble in water at room temperature up to 1.45 M. The pH of this solution will be approximately 10 to 11 and will be rather viscous.

Symbol	GHS05,GHS07,GHS08
Signal word	Danger
Hazard statements	H302 + H332 - H318 - H373
Precautionary statements	P260 - P280 - P301 + P312 + P330 - P305 + P351 + P338 + P310
Hazard Codes	Xn
Risk Statements	22-41
Safety Statements	26-39-46
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (KT)
UNSPSC	12352202