Ethylenediaminetetraacetic acid tetrasodium salt hydrate
SIGMA/03699 - BioUltra, ≥99.0% (KT)
Synonym: EDTA tetrasodium salt; Edathamil; Tetrasodium ethylenediaminetetraacetate hydrate
CAS Number: 194491-31-1
Empirical Formula (Hill Notation): C10H12N2Na4O8 · xH2O
Molecular Weight: 380.17 (anhydrous basis)
EC Number: 200-573-9
MDL Number: MFCD00150025
Linear Formula: (NaOOCCH2)2NCH2CH2N(CH2COONa)2 · xH2O
Product Type: Chemical
λ | 0.1 M in H2O |
anion traces | chloride (Cl-): ≤100 mg/kg |
sulfate (SO42-): ≤100 mg/kg | |
assay | ≥99.0% (KT) |
cation traces | Al: ≤5 mg/kg |
As: ≤0.1 mg/kg | |
Ba: ≤5 mg/kg | |
Bi: ≤5 mg/kg | |
Ca: ≤10 mg/kg | |
Cd: ≤5 mg/kg | |
Co: ≤5 mg/kg | |
Cr: ≤5 mg/kg | |
Cu: ≤5 mg/kg | |
Fe: ≤5 mg/kg | |
K: ≤50 mg/kg | |
Li: ≤5 mg/kg | |
Mg: ≤5 mg/kg | |
Mn: ≤5 mg/kg | |
Mo: ≤5 mg/kg | |
Ni: ≤5 mg/kg | |
Pb: ≤5 mg/kg | |
Sr: ≤5 mg/kg | |
Zn: ≤5 mg/kg | |
form | powder |
impurities | insoluble matter, passes filter test |
InChI | 1S/C10H16N2O8.4Na.H2O/c13 |
InChI key | GXFFSKMBMYHTAE-UHFFFAOYSA |
pH | 11.0-12.0 (25 °C, 0.1 M in H2O) |
product line | BioUltra |
Quality Level | 200 |
reaction suitability | reagent type: chelator |
SMILES string | O.[Na+].[Na+].[Na+].[Na+] |
solubility | H2O: 0.1 M at 20 °C, clear, colorless |
UV absorption | λ: 260 nm Amax: ≤1.5 |
λ: 280 nm Amax: ≤0.06 |
Application: | EDTA is an inhibitor of metalloproteases, at effective concentrations of 1-10 mM. EDTA acts as a chelator of the zinc ion in the active site of metalloproteases, and can also inhibit other metal ion-dependent proteases such as calcium-dependent cysteine proteases. For use as an anticoagulant, disodium or tripotassium salts of EDTA are most commonly used. The optimal concentration is 1.5 mg per ml of blood. |
Preparation Note: | Soluble in water at room temperature up to 1.45 M. The pH of this solution will be approximately 10 to 11 and will be rather viscous. |
Symbol | GHS05,GHS07,GHS08 |
Signal word | Danger |
Hazard statements | H302 + H332 - H318 - H373 |
Precautionary statements | P260 - P280 - P301 + P312 + P330 - P305 + P351 + P338 + P310 |
Hazard Codes | Xn |
Risk Statements | 22-41 |
Safety Statements | 26-39-46 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99.0% (KT) |
UNSPSC | 12352202 |