Methylene Blue solution

SIGMA/03978 - for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Synonym: Methylene blue stain; Ehrlich’s reagent III; Loeffler’s Methylene Blue

CAS Number: 61-73-4
Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S
Molecular Weight: 319.85
MDL Number: MFCD00012111
Linear Formula: C₁₆H₁₈ClN₃S
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-03978-250ML	250 mL	$55.60 1/EAAdd To Favorites		Add To Favorites

ε_max	9.4 at 656-666 nm in water
application(s)	diagnostic assay manufacturing hematology histology
concentration	1.5% (1.5 g/ 100 mL)
density	1.0 g/mL at 20 °C
form	liquid
grade	for microscopy
InChI	1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
InChI key	CXKWCBBOMKCUKX-UHFFFAOYSA-M
quality	concentrated, aqueous solution
Quality Level	100
shelf life	~3 yr
SMILES string	[Cl-].CN(C)c1ccc2N=C3C=CC(C=C3Sc2c1)=[N+](/C)C
storage temp.	room temp

Application:	Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.
Application:	Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.
General description:	Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright’s stain and Jenner’s stain.
General description:	Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.