N-Ethylmaleimide

SIGMA/04259 - BioUltra, ≥99.0% (HPLC)

Synonym: NEM

CAS Number: 128-53-0
Empirical Formula (Hill Notation): C₆H₇NO₂
Molecular Weight: 125.13
EC Number: 204-892-4
MDL Number: MFCD00005509
Linear Formula: C₆H₇NO₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-04259-5G	5 g	$175.00 1/EAAdd To Favorites	Add To Favorites
45-04259-25G	25 g	$680.00 1/EAAdd To Favorites	Add To Favorites

absorption	cut-off at 382 nm in methanol at 1 M
anion traces	chloride (Cl^-): ≤50 mg/kg
	sulfate (SO₄^2-): ≤100 mg/kg
assay	≥99.0% (HPLC)
bp	210 °C (lit.)
cation traces	Al: ≤5 mg/kg
	Ba: ≤5 mg/kg
	Bi: ≤5 mg/kg
	Ca: ≤10 mg/kg
	Cd: ≤5 mg/kg
	Co: ≤5 mg/kg
	Cr: ≤5 mg/kg
	Cu: ≤5 mg/kg
	Fe: ≤5 mg/kg
	K: ≤50 mg/kg
	Li: ≤5 mg/kg
	Mg: ≤5 mg/kg
	Mn: ≤5 mg/kg
	Mo: ≤5 mg/kg
	Na: ≤50 mg/kg
	Ni: ≤5 mg/kg
	Pb: ≤5 mg/kg
	Sr: ≤5 mg/kg
	Zn: ≤5 mg/kg
ign. residue	≤0.05%
impurities	insoluble matter, passes filter test
InChI	1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3
InChI key	HDFGOPSGAURCEO-UHFFFAOYSA-N
mp	43-46 °C (lit.)
	44-47 °C
product line	BioUltra
Quality Level	200
SMILES string	CCN1C(=O)C=CC1=O
solubility	methanol: 1 M at 20 °C, clear, colorless
storage temp.	2-8°C

Application:	N-Ethylmaleimide (NEM) has been used: • to prepare NEM-modified myosin beads • to block unmodified free thiols in samples • as a supplement in IP lysis/wash buffer for in vitro SUMOylation assay
Application:	Reagent for the covalent modification of cysteine residues in proteins.
Biochem/physiol Actions:	Augments currents from native M-channels in sympathetic neurons and acts as an opener for KCNQ2, KCNQ4 and KCNQ5 channels.
Biochem/physiol Actions:	Sulfhydryl alkylating agent that inactivates NADP-dependent isocitrate dehydrogenase and many endonucleases.

Symbol	GHS05,GHS06
Signal word	Danger
Hazard statements	H300 - H311 - H314 - H317
Precautionary statements	P260 - P280 - P301 + P310 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310
Hazard Codes	T+
Risk Statements	21-28-34-43
Safety Statements	26-28-36/37/39-45
RIDADR	UN 2928 8(6.1) / PGII
WGK Germany	WGK 3
Flash Point(F)	163.2 °F
Flash Point(C)	72.9 °C

Purity	≥99.0% (HPLC)
bp	210 °C (lit.)
mp	43-46 °C (lit.); 44-47 °C
Storage Temp.	2-8°C
UNSPSC	12352106