Phalloidin–Atto 665

SIGMA/04497 - suitable for fluorescence, ≥90% (HPLC)

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-04497-10NMOL	10 nmol	$630.00 1/EAAdd To Favorites	Add To Favorites

assay	≥90% (HPLC)
fluorescence	λ_ex 663 nm; λ_em 684 nm in 0.1 M phosphate pH 7.0
manufacturer/tradename	ATTO-TEC GmbH
Quality Level	100
storage temp.	−20°C
suitability	suitable for fluorescence

General description:

Atto 665 Phalloidin is a new fluorescent label closely related to Atto 647N. As such the dye shows an extraordinarily high fluorescence quantum yield, excellent thermal and photo-stability, outstanding ozone resistance, and very little triplet formation. Atto 665 Phalloidin is a cationic dye (charge +1). In common with most Atto-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications.
Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10^-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

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Legal Information:

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352116