Nitrilase
SIGMA/04529 - recombinant, expressed in E. coli, ≥2.0 U/mg
Synonym: Nitrile aminohydrolase
CAS Number: 9024-90-2
MDL Number: MFCD04040969
Product Type: Chemical
| form | crystals |
| powder | |
| mol wt | 41 kDa |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| specific activity | ≥2.0 U/mg |
| storage temp. | 2-8°C |
| Application: | Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies. |
| Biochem/physiol Actions: | Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity. |
| General description: | Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE). |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Unit Definition: | 1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2.0 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.5.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |