Agarose

SIGMA/05066 - High EEO, for molecular biology

Synonym: 3,6-Anhydro-α-L-galacto-β-D-galactan; Agarose HE

CAS Number: 9012-36-6
EC Number: 232-731-8
MDL Number: MFCD00081294
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-05066-50G	50 g	$216.00 1/EAAdd To Favorites	Add To Favorites
45-05066-500G	500 g	$1360.00 1/EAAdd To Favorites	Add To Favorites

anion traces	chloride (Cl^-): ≤0.3%
	sulfate (SO₄^2-): ≤0.6%
cation traces	Ca: ≤0.05%
	Cd: ≤0.001%
	Co: ≤0.001%
	Cr: ≤0.001%
	Cu: ≤0.001%
	Fe: ≤0.001%
	K: ≤0.05%
	Mg: ≤0.001%
	Mn: ≤0.001%
	Na: ≤0.5%
	Ni: ≤0.001%
	Pb: ≤0.001%
	Zn: ≤0.001%
EEO	0.23-0.27
form	powder
gel strength	≥1500 g/cm²
grade	for molecular biology
ign. residue	≤1%
impurities	DNases, none detected
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key	MJQHZNBUODTQTK-WKGBVCLCSA-N
loss	≤10% loss on drying
Quality Level	100
transition temp	gel point 34-37 °C (1.5% solution)

Analysis Note:	The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application:	Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.
Biochem/physiol Actions:	Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.
Packaging:	Packaging 50, 500 g in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable