Bovine Serum Albumin

SIGMA/05470 - lyophilized powder, crystallized, ≥98.0% (GE)

Synonym: Albumin bovine serum; BSA; Bovine albumin

CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-05470-5G	5 g	$419.00 1/EAAdd To Favorites	Add To Favorites
45-05470-25G	25 g	$1240.00 1/EAAdd To Favorites	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 05470-5G

anion traces	chloride (Cl^-): ≤2000 ppm
	sulfate (SO₄^2-): ≤1000 ppm
assay	≥98.0% (GE)
biological source	bovine
cation traces	Ca: ≤500 ppm
	Cd: ≤5 ppm
	Co: ≤5 ppm
	Cr: ≤100 ppm
	Cu: ≤20 ppm
	Fe: ≤20 ppm
	K: ≤50 ppm
	Mg: ≤500 ppm
	Mn: ≤5 ppm
	Na: ≤7000 ppm
	Ni: ≤5 ppm
	Pb: ≤5 ppm
	Zn: ≤10 ppm
form	lyophilized powder
mol wt	~66 kDa
packaging	glass bottle of
pH	4.8-7.5
Quality Level	200
solubility	H₂O: slightly soluble
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable
	electron microscopy: suitable
UniProt accession no.	P02769

Application:	Albumin from bovine serum can be used for Electron and Immunoelectron microscopy. It can also be used for isolating and culturing neonatal rat cardiac ventricular myocytes.
Application:	Bovine serum albumin has been used to quantify proteins in raw enzyme extracts. It has also been used as a blocking agent for mitochondrial imaging.
Biochem/physiol Actions:	Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also trigger insulin dependent diabetes mellitus.
Biochem/physiol Actions:	Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Other Notes:	Review
Other Notes:	Sales restrictions may apply
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.
Preparation Note:	Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98.0% (GE)
Storage Temp.	2-8°C
UNSPSC	12352202