Bovine Serum Albumin
SIGMA/05470 - lyophilized powder, crystallized, ≥98.0% (GE)
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 05470-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 05470-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 05470-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 05470-25MG
| anion traces | chloride (Cl-): ≤2000 ppm |
| sulfate (SO42-): ≤1000 ppm | |
| assay | ≥98.0% (GE) |
| biological source | bovine |
| cation traces | Ca: ≤500 ppm |
| Cd: ≤5 ppm | |
| Co: ≤5 ppm | |
| Cr: ≤100 ppm | |
| Cu: ≤20 ppm | |
| Fe: ≤20 ppm | |
| K: ≤50 ppm | |
| Mg: ≤500 ppm | |
| Mn: ≤5 ppm | |
| Na: ≤7000 ppm | |
| Ni: ≤5 ppm | |
| Pb: ≤5 ppm | |
| Zn: ≤10 ppm | |
| form | lyophilized powder |
| mol wt | ~66 kDa |
| packaging | glass bottle of |
| pH | 4.8-7.5 |
| Quality Level | 200  |
| solubility | H2O: slightly soluble |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| electron microscopy: suitable | |
| UniProt accession no. | P02769  |
| Application: | Albumin from bovine serum can be used for Electron and Immunoelectron microscopy. It can also be used for isolating and culturing neonatal rat cardiac ventricular myocytes. |
| Application: | Bovine serum albumin has been used to quantify proteins in raw enzyme extracts. It has also been used as a blocking agent for mitochondrial imaging. |
| Biochem/physiol Actions: | Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also trigger insulin dependent diabetes mellitus. |
| Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
| Other Notes: | Review |
| Other Notes: | Sales restrictions may apply |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98.0% (GE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |