Atto 655-Biotin

SIGMA/06966 - BioReagent, suitable for fluorescence, ≥90% (HPCE)

Synonym: Biotin-Atto 655

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-06966-1MG-F	1 mg	$367.00 1/EAAdd To Favorites	Add To Favorites

λ	in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)
assay	≥90% (HPCE)
fluorescence	λ_ex 655 nm; λ_em 680 nm in 0.1 M phosphate pH 7.0
form	powder
manufacturer/tradename	ATTO-TEC GmbH
product line	BioReagent
Quality Level	100
solubility	acetonitrile: soluble
	DMF: soluble
	DMSO: soluble
storage temp.	−20°C
suitability	suitable for fluorescence
transmittance	254 nm
	655 nm

Application:

Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.

Legal Information:

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90% (HPCE)
Storage Temp.	−20°C
UNSPSC	12352116