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Atto 655-Biotin

SIGMA/06966 - BioReagent, suitable for fluorescence, ≥90% (HPCE)

Synonym: Biotin-Atto 655

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-06966-1MG-F 1 mg
$354.00
1/EA
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λ in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)
assay ≥90% (HPCE)
fluorescence λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
form powder
manufacturer/tradename ATTO-TEC GmbH
product line BioReagent
Quality Level 100 
solubility acetonitrile: soluble
  DMF: soluble
  DMSO: soluble
storage temp. −20°C
suitability suitable for fluorescence
transmittance 254 nm
  655 nm
Application: Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.
Legal Information: This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥90% (HPCE)
Storage Temp. −20°C
UNSPSC 12352200

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