Atto 655-Biotin
SIGMA/06966 - BioReagent, suitable for fluorescence, ≥90% (HPCE)
Synonym: Biotin-Atto 655
Product Type: Chemical
| λ | in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm) |
| assay | ≥90% (HPCE) |
| fluorescence | λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0 |
| form | powder |
| manufacturer/tradename | ATTO-TEC GmbH |
| product line | BioReagent |
| Quality Level | 100  |
| solubility | acetonitrile: soluble |
| DMF: soluble | |
| DMSO: soluble | |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| transmittance | 254 nm |
| 655 nm |
| Application: | Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface. |
| Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPCE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |