Phalloidin–Atto 740
SIGMA/07373 - BioReagent, suitable for fluorescence
Synonym: Atto 740–Phalloidin
Product Type: Chemical
| assay | ≥90.0% (HPLC) |
| fluorescence | λex 750 nm; λem 764 nm in 0.1 M phosphate pH 7.0 |
| form | powder |
| manufacturer/tradename | ATTO-TEC GmbH |
| product line | BioReagent |
| Quality Level | 100  |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| transmittance | 254 nm |
| 740 nm | |
| UV absorption | λ: 739 -745 nm Amax |
| General description: | Atto 740 belongs to a new generation of fluorescent labels for the near infrared spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the dye are strong absorption and good fluorescence as well as excellent thermal and photo-stability. Atto 740 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. Atto 740 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the reaction. Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin. find more information here |
| Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90.0% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |