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6-Aminofluorescein

SIGMA/07985 - BioReagent, suitable for fluorescence, ~95% (HPLC)

Synonym: Fluoresceinamine isomer II

CAS Number: 51649-83-3
Empirical Formula (Hill Notation): C20H13NO5
Molecular Weight: 347.32
EC Number: 257-334-7
MDL Number: MFCD00005051
Linear Formula: C20H13NO5
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-07985-1G 1 g
$172.00
1/EA
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45-07985-5G 5 g
$676.00
1/EA
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assay ~95% (HPLC)
fluorescence λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0
form powder
impurities ≤5% 5-aminofluorescein
InChI 1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2
InChI key YOAWSYSKQHLFPM-UHFFFAOYSA-N
mp 285 °C (dec.) (lit.)
product line BioReagent
Quality Level 100 
SMILES string Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
solubility acetone: soluble
  DMSO: soluble
  methanol: soluble
suitability suitable for fluorescence
Application: 6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes’. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.
Other Notes: Note: Do not confuse with fluorescamine.
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ~95% (HPLC)
mp 285 °C (dec.) (lit.)
UNSPSC 12352200

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