6-Aminofluorescein
SIGMA/07985 - BioReagent, suitable for fluorescence, ~95% (HPLC)
Synonym: Fluoresceinamine isomer II
CAS Number: 51649-83-3
Empirical Formula (Hill Notation): C20H13NO5
Molecular Weight: 347.32
EC Number: 257-334-7
MDL Number: MFCD00005051
Linear Formula: C20H13NO5
Product Type: Chemical
assay | ~95% (HPLC) |
fluorescence | λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0 |
form | powder |
impurities | ≤5% 5-aminofluorescein |
InChI | 1S/C20H13NO5/c21-10-1-4-1 |
InChI key | YOAWSYSKQHLFPM-UHFFFAOYSA |
mp | 285 °C (dec.) (lit.) |
product line | BioReagent |
Quality Level | 100 |
SMILES string | Nc1ccc2C(=O)OC3(c4ccc(O)c |
solubility | acetone: soluble |
DMSO: soluble | |
methanol: soluble | |
suitability | suitable for fluorescence |
Application: | 6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes’. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein. |
Other Notes: | Note: Do not confuse with fluorescamine. |
Hazard Codes | Xi |
Risk Statements | 36/37/38 |
Safety Statements | 26-36 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ~95% (HPLC) |
mp | 285 °C (dec.) (lit.) |
UNSPSC | 12352200 |