6-Aminofluorescein

SIGMA/07985 - BioReagent, suitable for fluorescence, ~95% (HPLC)

Synonym: Fluoresceinamine isomer II

CAS Number: 51649-83-3
Empirical Formula (Hill Notation): C₂₀H₁₃NO₅
Molecular Weight: 347.32
EC Number: 257-334-7
MDL Number: MFCD00005051
Linear Formula: C₂₀H₁₃NO₅
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-07985-1G	1 g	$178.00 1/EAAdd To Favorites	Add To Favorites
45-07985-5G	5 g	$700.00 1/EAAdd To Favorites	Add To Favorites

assay	~95% (HPLC)
fluorescence	λ_ex 490 nm; λ_em 520 nm in 0.1 M Tris pH 9.0
form	powder
impurities	≤5% 5-aminofluorescein
InChI	1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2
InChI key	YOAWSYSKQHLFPM-UHFFFAOYSA-N
mp	285 °C (dec.) (lit.)
product line	BioReagent
Quality Level	100
SMILES string	Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
solubility	acetone: soluble
	DMSO: soluble
	methanol: soluble
suitability	suitable for fluorescence

Application:

6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes’. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.

Other Notes:

Note: Do not confuse with fluorescamine.

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable