Isoamylase from Pseudomonas sp.
SIGMA/08124 - ammonium sulfate suspension, ≥10,000,000 units/mg protein
MDL Number: MFCD00131418
Product Type: Chemical
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| form | ammonium sulfate suspension |
| Quality Level | 100  |
| specific activity | ≥10,000,000 units/mg protein |
| storage temp. | 2-8°C |
| Analysis Note: | ≥10 MU/mg protein |
| Application: | Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions. |
| Biochem/physiol Actions: | Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch. |
| Biochem/physiol Actions: | Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. |
| Unit Definition: | One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10,000,000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.68 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |