7-Amino-4-methyl-3-coumarinylacetic acid
SIGMA/08445 - BioReagent, suitable for fluorescence, ~90% (HPLC)
Synonym: 2H-
CAS Number: 106562-32-7
Empirical Formula (Hill Notation): C12H11NO4
Molecular Weight: 233.22
MDL Number: MFCD00081051
Linear Formula: C12H11NO4
Product Type: Chemical
| assay | ~90% (HPLC) |
| fluorescence | λex 350 nm; λem 433 nm in methanol |
| form | powder |
| InChI | 1S/C12H11NO4/c1-6-8-3-2-7 |
| InChI key | QEQDLKUMPUDNPG-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | CC1=C(CC(O)=O)C(=O)Oc2cc( |
| solubility | aqueous base: soluble |
| DMF: soluble | |
| DMSO: soluble | |
| suitability | suitable for fluorescence |
| Application: | AMCA is a useful agent for labeling proteins. Demonstrates a characteristic absorption peak at 345 nm with a fluorescence emission at 440-460 nm (blue region); however, when conjugated with proteins the absorption peak shifts to 355 nm with a fluorescence emission at 440-460 nm. It has a high fluorescence yield with a sufficient Stokes shift (100 nm) and is relatively photostable . Relatively easy to activate and couple to the N-terminal amino group of a peptide resulting in an acid stable amide bond . |
| Other Notes: | Useful blue fluorophore for immunofluorescence, λabs ~350 nm, λem ~440 nm |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ~90% (HPLC) |
| UNSPSC | 12352106 |