7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester

SIGMA/08450 - BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym: 7-(Dimethylamino)coumarin-4-acetic acid-NHS; 7-Amino-4-methyl-3coumarinylacetyl; N-Succinimidyl 7-amino-4-methyl-3-coumarinylacetate; AMCA-NHS; Succinimidyl-7-amino-4-methylcoumarin-3-acetate

CAS Number: 113721-87-2
Empirical Formula (Hill Notation): C₁₆H₁₄N₂O₆
Molecular Weight: 330.29
MDL Number: MFCD00036170
Linear Formula: C₁₆H₁₄N₂O₆
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-08450-5MG	5 mg	$313.00 1/EAAdd To Favorites	Add To Favorites

assay	≥90% (HPLC)
fluorescence	λ_ex 354 nm; λ_em 440 nm in methanol
InChI	1S/C16H14N2O6/c1-8-10-3-2-9(17)6-12(10)23-16(22)11(8)7-15(21)24-18-13(19)4-5-14(18)20/h2-3,6H,4-5,7,17H2,1H3
InChI key	ILKQVTIMOGNSAS-UHFFFAOYSA-N
product line	BioReagent
Quality Level	100
SMILES string	CC1=C(CC(=O)ON2C(=O)CCC2=O)C(=O)Oc3cc(N)ccc13
solubility	DMF: soluble
	DMSO: soluble
storage temp.	−20°C
suitability	suitable for fluorescence

Application:	AMCA-NHS has been utilized in the development of a donor europium chelate fluorochrome for use in LRET based analyses by first reacting with an amino group and then with DTPA anhydride . Utilized in FRET based studies, acting as the donor dye and can be paired with fluorescein-5-isothiocyanate as the acceptor dye . A blue fluorescent dye reactive towards primary amine groups on proteins, peptides, antibodies, and other biomolecules (at pH 7.0-9.0).
Other Notes:	Labeling of lysyl residues in proteins
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

Symbol	GHS07
Signal word	Warning
Hazard statements	H315 - H319 - H335
Precautionary statements	P302 + P352 - P305 + P351 + P338
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352108