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7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester

SIGMA/08450 - BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym: 7-(Dimethylamino)coumarin-4-acetic acid-NHS; 7-Amino-4-methyl-3coumarinylacetyl; N-Succinimidyl 7-amino-4-methyl-3-coumarinylacetate; AMCA-NHS; Succinimidyl-7-amino-4-methylcoumarin-3-acetate

CAS Number: 113721-87-2
Empirical Formula (Hill Notation): C16H14N2O6
Molecular Weight: 330.29
MDL Number: MFCD00036170
Linear Formula: C16H14N2O6
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-08450-5MG 5 mg
 $313.00
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assay ≥90% (HPLC)
fluorescence λex 354 nm; λem 440 nm in methanol
InChI 1S/C16H14N2O6/c1-8-10-3-2-9(17)6-12(10)23-16(22)11(8)7-15(21)24-18-13(19)4-5-14(18)20/h2-3,6H,4-5,7,17H2,1H3
InChI key ILKQVTIMOGNSAS-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string CC1=C(CC(=O)ON2C(=O)CCC2=O)C(=O)Oc3cc(N)ccc13
solubility DMF: soluble
  DMSO: soluble
storage temp. −20°C
suitability suitable for fluorescence
Application: AMCA-NHS has been utilized in the development of a donor europium chelate fluorochrome for use in LRET based analyses by first reacting with an amino group and then with DTPA anhydride . Utilized in FRET based studies, acting as the donor dye and can be paired with fluorescein-5-isothiocyanate as the acceptor dye . A blue fluorescent dye reactive towards primary amine groups on proteins, peptides, antibodies, and other biomolecules (at pH 7.0-9.0).
Other Notes: Labeling of lysyl residues in proteins
Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
Purity ≥90% (HPLC)
Storage Temp. −20°C
UNSPSC 12352108

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