6-Carboxy-fluorescein diacetate N-succinimidyl ester
SIGMA/08951 - for fluorescence, ≥90.0% (HPLC)
Synonym: 6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester
CAS Number: 150206-15-8
Empirical Formula (Hill Notation): C29H19NO11
Molecular Weight: 557.46
MDL Number: MFCD00269772
Linear Formula: C29H19NO11
Product Type: Chemical
assay | ≥90.0% (HPLC) |
fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase) |
grade | for fluorescence |
InChI | 1S/C29H19NO11/c1-14(31)37 |
InChI key | JGPOSNWWINVNFV-UHFFFAOYSA |
Quality Level | 100 |
SMILES string | CC(=O)Oc1ccc2c(Oc3cc(OC(C |
solubility | DMF: soluble |
DMSO: soluble | |
storage temp. | −20°C |
Application: | The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells. |
Other Notes: | Reagent for continuously determining the intracellular pH in bacteria by fluorometry; labeling human intervertebral disk cells in vitro for fluorescence microscopy |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥90.0% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |