Ammonium sulfate

SIGMA/09978 - BioUltra, ≥99.0% (T)

Synonym: Mascagnite

CAS Number: 7783-20-2
Empirical Formula (Hill Notation): H₈N₂O₄S
Molecular Weight: 132.14
EC Number: 231-984-1
MDL Number: MFCD00003391
Linear Formula: (NH₄)₂SO₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-09978-500G	500 g	$137.00 1/EAAdd To Favorites	Add To Favorites
45-09978-1KG	1 kg	$231.00 1/EAAdd To Favorites	Add To Favorites
45-09978-2.5KG	2.5 kg	$467.00 1/EAAdd To Favorites	Add To Favorites

λ	1 M in H₂O
anion traces	chloride (Cl^-): ≤5 mg/kg
	nitrate (NO₃^-): ≤50 mg/kg
	phosphate (PO₄^3-): ≤5 mg/kg
assay	≥99.0% (T)
cation traces	Al: ≤5 mg/kg
	As: ≤0.2 mg/kg
	Ba: ≤5 mg/kg
	Bi: ≤5 mg/kg
	Ca: ≤10 mg/kg
	Cd: ≤1 mg/kg
	Co: ≤5 mg/kg
	Cr: ≤5 mg/kg
	Cu: ≤2 mg/kg
	Fe: ≤2 mg/kg
	K: ≤50 mg/kg
	Li: ≤5 mg/kg
	Mg: ≤50 mg/kg
	Mn: ≤5 mg/kg
	Mo: ≤5 mg/kg
	Na: ≤50 mg/kg
	Ni: ≤5 mg/kg
	Pb: ≤2 mg/kg
	Sr: ≤5 mg/kg
	Zn: ≤1 mg/kg
density	1.77 g/mL at 25 °C (lit.)
form	crystals
ign. residue	≤0.01% (as SO₄)
impurities	insoluble matter, passes filter test
InChI	1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)
InChI key	BFNBIHQBYMNNAN-UHFFFAOYSA-N
loss	≤0.1% loss on drying, 110 °C
mp	>280 °C (dec.) (lit.)
pH	5.0-6.0 (25 °C, 1 M in H₂O)
product line	BioUltra
Quality Level	200
SMILES string	N.N.OS(O)(=O)=O
solubility	H₂O: 1 M at 20 °C, clear, colorless
UV absorption	λ: 260 nm A_max: ≤0.037
	λ: 280 nm A_max: ≤0.030

Application:	Ammonium sulfate has been used: • to prepare mobile phase A for hydrophobic interaction chromatography (HIC) • as reference material for the thermal decomposition of NH₄NO₃ (S1–S6) thermal decomposition to N₂O (S1-N₂O–S6-N₂O) • to prepare the stock solution for M63 medium • as a reagent in molecular biology and chromatography • in the precipitation and fractionation of proteins • in the purification of antibodies • for the crystallographic analysis of nucleic acids and proteins • in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography • for Sepharose chromatography of tRNAs using reverse salt gradients • in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis • HPLC analysis of phospholipids
Biochem/physiol Actions:	Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.
General description:	Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.
Legal Information:	Sepharose is a trademark of Cytiva
Other Notes:	The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose^™ chromatography of tRNAs using reverse salt gradients.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (T)
mp	>280 °C (dec.) (lit.)
Density	1.77 g/mL at 25 °C (lit.)
UNSPSC	12352314