Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, neonatal

SIGMA/100-05N

Product Type: Product-on-demand

Catalog Number	PKG Qty.	Price	Quantity


45-100-05N	1 each	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

Cell Applications : CADMEC/HMVEC monolayer (left), vessel-like structures formed in an angiogenesis assay (middle) and immunolabeled for CD31/PECAM (right, green). Nuclei are visualized with PI (right, red).

biological source	human neonatal foreskin or adult skin capillaries (normal)
growth mode	Adherent
karyotype	2n = 46
manufacturer/tradename	Cell Applications, Inc
morphology	Endothelial
packaging	pkg of 500,000 cells
Quality Level	100
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	Endothelial vessel function, cell signaling, angiogenesis
Cell Line Origin:	Skin
Components:	Cell Basal Medium containing 10% FBS & 10% DMSO
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description:	Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. CADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence. Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).
Preparation Note:	• 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 16 doublings
Subculture Routine:	Please refer to the CADMEC/HMVEC Culture Protocol .