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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, neonatal

SIGMA/100-05N

Product Type: Product-on-demand

Catalog Number PKG Qty. Price Quantity
45-100-05N 1 each
$800.00
1/EA
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Cell Applications : CADMEC/HMVEC monolayer (left), vessel-like structures formed in an angiogenesis assay (middle) and immunolabeled for CD31/PECAM (right, green). Nuclei are visualized with PI (right, red).

 

biological source human neonatal foreskin or adult skin capillaries (normal)
growth mode Adherent
karyotype 2n = 46
manufacturer/tradename Cell Applications, Inc
morphology Endothelial
packaging pkg of 500,000 cells
Quality Level 100 
shipped in dry ice
storage temp. −196°C
technique(s) cell culture | mammalian: suitable
Application: Endothelial vessel function, cell signaling, angiogenesis
Cell Line Origin: Skin
Components: Cell Basal Medium containing 10% FBS & 10% DMSO
Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description: Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

CADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence.

Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).
Preparation Note: • 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings
Subculture Routine: Please refer to the CADMEC/HMVEC Culture Protocol .
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −196°C
UNSPSC 41106514

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