SerBob cell line human

SIGMA/10021101

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-10021101-1VL	1 vial	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

biological source	human prostate
form	liquid
growth mode	Adherent
karyotype	4n (67 to 88)
morphology	Cobblestone
products	More differentiated than Bob with increased CD24, PAP, PSCA expression (prostate differentiation markers). 10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour. More invasive than Bob.
Quality Level	100
receptors	CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	Prostate cancer research including drug testing.
Cell Line Description:	First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. SerBob grows as a monolayer culture with limited differentiation. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Cell Line Origin:	Human prostate cancer spontaneously immortalized
Culture Medium:	Keratinocyte-SFM (Invitrogen, catalogue no 37010-022, supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor + 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
DNA Profile:	STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 9,14 D16S539: 10,12 D5S818: 13 D7S820: 10,11 THO1: 9 TPOX: 8,10 vWA: 17
Other Notes:	Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Subculture Routine:	Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. At confluence 5-7 x 104 cells/cm² can be expected.