α-Amylase from Aspergillus oryzae
SIGMA/10065 - powder, ~30 U/mg
Synonym: 1,4-α-D-Glucan-glucanohydrolase
EC Number: 232-588-1
MDL Number: MFCD00081319
Product Type: Chemical
| form | powder |
| mol wt | Mr ~51000 |
| Quality Level | 100  |
| specific activity | ~30 U/mg |
| storage temp. | 2-8°C |
| Application: | α-Amylase has been used: • as a control enzyme in agar plate-based and carboxymethylcellulose-ba • for the hydrolysis of starch to explore the the role of wheat starch in frozen dough • to inhibit Staphylococcus aureus biofilms |
| Biochem/physiol Actions: | Aspergillus oryzae α-amylase (Ao α-amylase) enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins. |
| General description: | Microbial amylases are exoenzymes and are used in several industrial applications, such as production of bread, maltose syrups, and fermentation of soya sauce, miso etc. α-Amylase isolated from Aspergillus oryzae has been found to have molecular weights of 51kDa (sedimentation and diffusion) and 49kDa (gel filtration). |
| Other Notes: | For the determination of fats in food; Application in (selective) hydrolysis/condensation of glycosidic bonds. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Unit Definition: | 1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate). |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H334 |
| Precautionary statements | P261 - P264 - P270 - P284 - P301 + P312 - P304 + P340 + P312 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-24-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ~30 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |