CCB cell line from carp
SIGMA/10072802
Product Type: Chemical
biological source | fish brain |
growth mode | Adherent |
karyotype | Not specified |
morphology | Squamous |
products | Not specified |
Quality Level | 100 |
receptors | Not specified |
shipped in | dry ice |
storage temp. | −196°C |
technique(s) | cell culture | mammalian: suitable |
Cell Line Description: | The CCB cell line was developed from Common carp (Cyprinus carpio carpio) brain. |
Cell Line Origin: | Common carp brain cell line, koi herpesvirus isolation |
Culture Medium: | EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). 10 mM HEPES can be included if a CO2 incubator is not available. |
Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
Subculture Routine: | Split sub-confluent cultures (70-80%) 1:4 to 1:6, i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25 °C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −196°C |
UNSPSC | 12352200 |