COCA cell line from mouse
SIGMA/10112001
Synonym: COCA cells
Product Type: Chemical
| biological source | mouse skin |
| growth mode | Adherent |
| karyotype | See Segrelles et al 2011 |
| morphology | Epithelial |
| products | See Segrelles et al 2011 |
| Quality Level | 100  |
| receptors | Not specified |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | For complete product information, please see ECACC  |
| Cell Line Description: | COCA, a murine epidermal cell line was developed by serially passaging keratinocytes from the back skin of adult C57BL/DBA mice. Culture of the COCA cells is in fully defined media without requirement for feeder layer or other coating. COCA retains its ability to differentiate and stratify in response to increased calcium concentrations thus providing an excellent experimental system for in vitro (conventional and 3D epidermal cell cultures) and in vivo (skin regeneration) skin modelling. |
| Cell Line Origin: | Mouse epidermal keratinocyes, differentiation |
| Culture Medium: | CnT-07 media This defined medium contains low calcium (0.07mM). To promote good cell attachment post trypsinisation, an 8-14 hours incubation in CnT-07 medium containing 0.2mM CaCl2 is necessary. Differentiation can be initiated by transferring a confluent monolayer from 0.07mM calcium to high calcium (1.2mM). |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from HPA Culture Collections, supplied by us, are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 0.5-1.5 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remov |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |