COCA cell line from mouse

SIGMA/10112001

Synonym: COCA cells

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-10112001-1VL	1 vial	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

biological source	mouse skin
growth mode	Adherent
karyotype	See Segrelles et al 2011
morphology	Epithelial
products	See Segrelles et al 2011
Quality Level	100
receptors	Not specified
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	For complete product information, please see ECACC
Cell Line Description:	COCA, a murine epidermal cell line was developed by serially passaging keratinocytes from the back skin of adult C57BL/DBA mice. Culture of the COCA cells is in fully defined media without requirement for feeder layer or other coating. COCA retains its ability to differentiate and stratify in response to increased calcium concentrations thus providing an excellent experimental system for in vitro (conventional and 3D epidermal cell cultures) and in vivo (skin regeneration) skin modelling.
Cell Line Origin:	Mouse epidermal keratinocyes, differentiation
Culture Medium:	CnT-07 media This defined medium contains low calcium (0.07mM). To promote good cell attachment post trypsinisation, an 8-14 hours incubation in CnT-07 medium containing 0.2mM CaCl2 is necessary. Differentiation can be initiated by transferring a confluent monolayer from 0.07mM calcium to high calcium (1.2mM).
Other Notes:	Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Other Notes:	Cultures from HPA Culture Collections, supplied by us, are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Subculture Routine:	Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 0.5-1.5 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remov