P4E6 cell line

SIGMA/10112301

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-10112301-1VL	1 vial	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

biological source	human prostate
growth mode	Not specified
karyotype	Not specified
morphology	Epithelial
products	Stains positive for pancytokeratin, cytokeratin 8, vimentin (weak) and chromogranin A. Prostate markers expressed include prostate specific antigen (PSA) prostate specific membrane antigen (PSMA), negative for expression of androgen receptor (AR).
Quality Level	100
receptors	Not specified
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Cell Line Origin:	Prostate cancer well differentiated, early stage
Culture Medium:	Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Disclaimer:	This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. Additionally, any recipient wanting to use the cell line in the field of vaccines for the treatment or prevention of prostate cancer should first obtain permission from GemVax & KAEL Co., Ltd. Contact : Dr. Eujin Hyun, Head of R&D Department (eujinhyun@kaelgemvax.com).
DNA Profile:	STR-PCR Data: Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 7,8 THO1: 7,9.3 TPOX: 8 vWA: 17,19
Other Notes:	Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Subculture Routine:	Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.