Human Epidermal Keratinocytes: HEK, neonatal
SIGMA/102-05N
Synonym: HEK cells
Product Type: Product-on-demand
Cell Applications : (A) Human Epidermal Keratinocytes, HEK, monolayer; (B) HEK immunolabeled for cytokeratin 18 (red) and e-cadherin (green). Nuclei are visualized with DAPI (blue); (C) HEK 3d skin model.
| biological source | human neonatal foreskin or adult skin (normal) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | Epidermal |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | allergies; cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response |
| Cell Line Origin: | Skin |
| Components: | Basal Medium containing 10% FBS & 10% DMSO |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation. HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection. When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies. |
| Preparation Note: | • Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 16 doublings |
| Subculture Routine: | Please refer to the HEK Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |