Human Epidermal Melanocytes: HEM, adult

SIGMA/104-05A

Synonym: HEM cells

Product Type: Product-on-demand

Catalog Number	PKG Qty.	Price	Quantity


45-104-05A	1 each	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

Cell Applications : Human Epidermal Melanocytes, HEM

biological source	human skin (normal abdominal)
growth mode	Adherent
karyotype	2n = 46
manufacturer/tradename	Cell Applications, Inc
morphology	Epidermal
packaging	pkg of 500,000 cells
Quality Level	100
relevant disease(s)	metabolic disorders
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	melanin production, melanocyte proliferation, differentiation
Cell Line Origin:	Skin
Components:	Basal Medium containing 30% FBS & 10% DMSO
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description:	Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.
Preparation Note:	• 2nd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO • Can be cultured at least 12 doublings
Subculture Routine:	Please refer to the HEM Culture Protocol .