Human Epidermal Melanocytes: HEM, neonatal
SIGMA/104-05N
Product Type: Product-on-demand
Cell Applications : Human Epidermal Melanocytes, HEM
| biological source | human neonatal foreskin (normal) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | Epidermal |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | metabolic disorders |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | melanin production, melanocyte proliferation, differentiation |
| Cell Line Origin: | Skin |
| Components: | Basal Medium containing 30% FBS & 10% DMSO |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia. |
| Preparation Note: | • 3rd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO • Can be cultured at least 12 doublings |
| Subculture Routine: | Please refer to the HEM Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |