Human Dermal Fibroblasts: HDF, adult
SIGMA/106-05A
Synonym: HDF cells
Product Type: Product-on-demand
Cell Applications : (A) Human Dermal Fibroblasts, HDF; (B) HDF immunolabeled for vimentin (red); (C) HDF stained for FSP (green).
| biological source | human neonatal foreskin or adult skin (normal) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | Fibroblast |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response |
| Cell Line Origin: | Skin |
| Components: | Fibroblast Basal Medium containing 10% FBS & 10% DMSO. |
| Culture Medium: | Fibroblast Growth Medium (116-500) all-in-one ready to use media |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013) |
| Preparation Note: | • Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO. • Can be cultured at least 16 doublings. |
| Subculture Routine: | Please refer to the HDF Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |