Human Dermal Fibroblasts: HDF, fetal

SIGMA/106-05F

Product Type: Product-on-demand

Catalog Number	PKG Qty.	Price	Quantity


45-106-05F	1 each	$677.00 1/EAAdd To Favorites		Add To Favorites

Cell Applications : (A) Human Dermal Fibroblasts, HDF; (B) HDF immunolabeled for vimentin (red); (C) HDF stained for FSP (green).

biological source	human neonatal foreskin or adult skin (normal)
growth mode	Adherent
karyotype	2n = 46
manufacturer/tradename	Cell Applications, Inc
morphology	Fibroblast
packaging	pkg of 500,000 cells
Quality Level	100
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response
Cell Line Origin:	Skin
Components:	Fibroblast Basal Medium containing 10% FBS & 10% DMSO
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description:	Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)
Preparation Note:	• Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 16 doublings
Subculture Routine:	Please refer to the HDF Culture Protocol .