Histopaque®-1077
SIGMA/10771 - sterile-filtered, density: 1.077
Product Type: Chemical
Histopaque®-1077 is suitable for use in hematological and histological applications. Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
Suitable for use in hematological and histological applications. Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| application(s) | hematology histology |
| density | 1.077 |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shelf life | Expiry date on the label. |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Histopaque-1077 has been used for:• human lymphocyte antigen (HLA) typing • initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes. • preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays. • isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) • isolation of human lymphocytes • isolation of breast cancer circulating tumor cells (CTCs) from the whole blood • Histopaque-1077 is used in conjunction with Histopaque-1119 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood |
| Biochem/physiol Actions: | Anticoagulated blood is layered onto Histopaque-1077. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment. Granulocytes become slightly hypertonic, which increases their sedimentation rate, resulting in pelleting at the bottom of the centrifuge tube. Lymphocytes and other mononuclear cells remain at the plasma/ Histopaque-1077 interface. |
| Features and Benefits: | Histopaque®-1077 offers numerous advantages, such as: • Optimal recovery of viable cells • Permits selective separation of blood cell types • Prevents cell distortion • Preserves cell viability • Minimal extraneous cell interference • Reproducible performance from batch to batch • Sterile-filtered and endotoxin-tested • Stable at 2-8°C for at least 2 years when protected from light |
| General description: | Histopaque-1077 is a sterile, endotoxin tested, ready-to-use medium. The Histopaque composition includes polysucrose and sodium diatrizoate, adjusted to a relative density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood or bone marrow. |
| Legal Information: | Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 - H350 |
| Precautionary statements | P202 - P261 - P272 - P280 - P302 + P352 - P308 + P313 |
| Hazard Codes | Xi |
| Risk Statements | 36/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.077 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |