TRIS hydrochloride
SIGMA/108319 - EMPROVE® EVOLVE
Synonym: Trizma® hydrochloride; TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
| biological source | synthetic |
| description | Manufactured under appropriate controls for use as a raw material in BioProcessing |
| foreign activity | Cytotoxicity, contains (tested) |
| DNase, contains (tested) | |
| NICKase, contains (tested) | |
| Protease, contains (tested) | |
| RNase, contains (tested) | |
| form | powder |
| impurities | Endotoxin, tested |
| Microbial, tested | |
| Trace Metals, tested | |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| pKa (25 °C) | 8.1 |
| product line | EMPROVE® EVOLVE |
| Quality Level | 400  |
| SMILES string | Cl.NC(CO)(CO)CO |
| suitability | suitable for manufacturing use |
| technique(s) | cell culture | mammalian: suitable |
| useful pH range | 7.0-9.0 |
| Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Application: | Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36. Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations. |
| Legal Information: | Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352200 |