FLO-1 cell line human
SIGMA/11012001
Synonym: FLO1 Cells
Product Type: Chemical
| biological source | human esophagus |
| growth mode | Adherent |
| karyotype | Not specified |
| morphology | Epithelial |
| products | P53 mutation c.830G>T |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | For complete product information, please see ECACC  |
| Cell Line Origin: | Distal oesophageal adenocarcinoma |
| Culture Medium: | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) |
| DNA Profile: | STR-PCR Data: Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 12,13 D5S818: 12,14 D7S820: 8 THO1: 6 TPOX: 9,11 vWA: 16 |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |