OACP4 C
SIGMA/11012005 - NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human gastric cardia, pleiomorphic: epithelial and fibroblastoid cells, cytoplasmic protrusions
Product Type: Chemical
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| biological source | human gastric cardia |
| growth mode | adherent |
| karyotype | Aneuploid 53-57 chromosomes |
| morphology | Pleiomorphic: both epithelial and fibroblastoid cells with cytoplasmic protrusions |
| packaging | pkg of vial of cells 11012005-1VL |
| tube of 5 μg 11012005-DNA-5UG | |
| Quality Level | 100  |
| technique(s) | cell culture | mammalian: suitable |
| Biochem/physiol Actions: | OACP4 C was established from a primary tumour located in gastric cardia of a 55 year-old Caucasian male. The patient showed lymph node involvement with pleural metastases. OACP4 C was found to be tumourigenic in NMRI (female nude mice or NOD-SCID mice. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino, et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer. |
| Biochem/physiol Actions: | STR-PCR Data: Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 12 D5S818: 9 D7S820: 9,11 THO1: 9 TPOX: 11 vWA: 16 |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Other Notes: | If use of this culture results in a scientific publication, it should be cited in the publication as: OACP4 C (ECACC 11012005) |
| Packaging: | NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. |
| Preparation Note: | RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442) |
| Preparation Note: | Split sub-confluent cultures (70-80%) up to 1:5 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106514 |